Bst2.0 WarmStartDNA Polymerase from New England Biolabs

Product Specs
ItemBst2.0 WarmStartDNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0538M
Quantity8000 units
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Simultaneous and rapid colorimetric detection of distinct miRNAs using Split-LAMPFrontiers in Bioengineering and BiotechnologyNovember 2, 2023Yi Jing Chua, Steven Poh Chuen Sim, Medha Shridharan, Yiqi Seow2.2 Primers (Integrated DNA Technologies),As stated in Section 2.2 "WarmStart Bst 2.0 (catalog number M0538M, New England Biolabs)",6.4 U Components in a LAMP reaction.

(2) Loop mediated isothermal amplification as a molecular diagnostic assay: Application and evaluation for detection of Enterohaemorrhagic Escherichia coli (O157:H7)Practical Laboratory MedicineNovember 1, 2023Degisew Yinur, Biniam Moges, Aliyi Hassen, Tesfaye Sisay TessemaMgSO4, 2.5 μl of 10X Bst ThermoPol reaction buffer, 8 U of Bst 2.0 DNA polymerase (NEW ENGLAND BioLABS #M0538), ddH2O, and 2 μL of target genomic DNA. The reaction mixture was incubated at 65 °C for 60 min in a

(3) The extinct Sicilian wolf shows a complex history of isolation and admixture with ancient dogsiScienceAugust 18, 2023Marta Maria Ciucani, Jazmín Ramos-Madrigal, Germán Hernández-Alonso, Alberto Carmagnini, Sabhrina Gita Aninta, Xin Sun, Camilla Hjorth Scharff-Olsen, Liam Thomas Lanigan, Ilaria Fracasso, Cecilie G. Clausen, et al.T4 DNA Polymerase,New England Biolabs Inc.,Cat#M0203S "BSt 2,0 warmstart polymerase",New England Biolabs Inc.,Cat#M0538S Phusion® High-Fidelity PCR Master Mix with HF buffer,New England Biolabs Inc.,Cat#M0531S DNeasy® Blood

(4) Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMPLab on a ChipJuly 25, 2023Daniel W. Weisgerber, Krzysztof Langer, Venice Servellita, Peng Xu, Charles Y. Chiu, Adam R. AbateB1003S), 1.875 mM dNTPs (ThermoFisher Scientific R1122), 8 U Bst 2.0 WarmStart DNA polymerase (NEB M0538L), 100 U SuperScript III reverse transcriptase (ThermoFisher Scientific 18 080 093) and the LAMP primer

(5) Ultrasensitive Isothermal Detection of SARS-CoV-2 Based on Self-Priming Hairpin-Utilized Amplification of the G-Rich SequenceAnalytical ChemistryDecember 20, 2022Yan Li, Hansol Kim, Yong Ju, Yeonkyung Park, Taejoon Kang, Dongeun Yong, Hyun Gyu Parkthe Korea Bank for Pathogenic Viruses (KBPV, Seoul, Korea). Bst 2.0 WarmStart DNA polymerase (Bst, M0538S), Nt.BstNBI (R0607S), NEBuffer 3.1 (B7203S), ThermoPol reaction buffer (B9004S), and deoxynucleotide (dNTP) solution mix

(6) Development of an optimized colorimetric RT-LAMP for SARS-CoV-2 assay with enhanced procedure controls for remote diagnosticsScientific ReportsDecember 11, 2022Bruna Winkert Raddatz, Edson Yu Sin Kim, Louise Matiê Imamura, Gisleine Jarenko Steil, Erika Bergamo Santiago, Santiago Pedro Timm Soares, Victor Henrique Alves Ribeiro, Bernardo Montesanti Machado de Almeida, Sergio Renato Rogal, Marcus Vinícius Mazega Figueredoconfigurations. Materials and methods Reagents and equipment Bst 2.0 WarmStart® DNA Polymerase (M0538), Antarctic Thermolabile UDG (M0372), WarmStart® RTx Reverse Transcriptase (M0380), as well as dNTPs

(7) METTL14 is required for exercise-induced cardiac hypertrophy and protects against myocardial ischemia-reperfusion injuryNature communicationsNovember 9, 2022Wang L, Wang J, Yu P, Feng J, Xu GE, Zhao X, Wang T, Lehmann HI, Li G, Sluijter JPG, et al.1 min, 50 °C for 1 min, 40 °C for 6 min)53,71. Then, 0.1 U Bst 2.0 WarmStart DNA polymerase (NEB, #M0538S), 5U SplintR ligase (NEB, #M0375S), 50μΜ dTTP (Diamond, #B110051-0250) and 1 mM ATP (NEB, #P0756S) was

(8) Polony gels enable amplifiable DNA stamping and spatial transcriptomics of chronic painCellNovember 8, 2022Xiaonan Fu, Li Sun, Runze Dong, Jane Y. Chen, Runglawan Silakit, Logan F. Condon, Yiing Lin, Shin Lin, Richard D. Palmiter, Liangcai Gubuffer,Qiagen,Cat#1034963 BSA,New England Biolabs,Cat#M9000S Bst 2.0 WarmStart DNA polymerase,New England Biolabs,Cat#M0538L Ultra II Q5 master mix,New England Biolabs,Cat#M0544L Thermolabile exonuclease I,New England Biolabs,Cat#M0568L Q5

(9) A method of large DNA fragment enrichment for nanopore sequencing in region 22q11.2Frontiers in GeneticsOctober 31, 2022Yu-Qing Lei, Liang-Pu Xu, Hua Cao, Xin-Rui Wangquickly placed on ice. After adding eight units of Bst 2.0 WarmStart DNA polymerase (NEB, Cat. no. M0538), the samples were incubated at 60°C for 10 min and 80°C for 20 min. Ten units of T7 exonuclease (NEB,

(10) Development and Clinical Validation of RT-LAMP-Based Lateral-Flow Devices and Electrochemical Sensor for Detecting Multigene Targets in SARS-CoV-2International Journal of Molecular SciencesOctober 28, 2022Apoorva Saxena, Pawankumar Rai, Srishti Mehrotra, Samiya Baby, Suman Singh, Vikas Srivastava, Smriti Priya, Sandeep K. Sharmabuffer, 6 mM of MgSO4, 1.4 mM of dNTPs, 800 mM of betaine, 8 U of Bst 2.0 WarmStart DNA polymerase (#M0538, New England Biolabs, Ipswich, MA, USA), 1.6 M of forward inner primer (FIP) and backward inner primer

(11) Point-of-care testing for COVID-19 using SHERLOCK diagnosticsmedRxivOctober 1, 2022Julia Joung et al.freeze-thaw cycles. For Step (2) - STOPCovid detection reaction: Bst 2.0 WarmStart® DNA Polymerase (M0538L), New England BioLabsWarmStart® RTx Reverse Transcriptase (M0380L), New England BioLabs10X Isothermal Amplification

(12) Functional inference of gene regulation using single-cell multi-omicsCell GenomicsSeptember 1, 2022Vinay K. Kartha et al.whilst minimizing the number of droplets with multiple beads to <10%. Furthermore, Bst 2.0 WarmStart (M0538S, NEB) was added to the droplet mix to perform temperature activated second strand synthesis in droplets. scATAC-seq

(13) Stimulation of adaptive gene amplification by origin firing under replication fork constraintNucleic Acids ResearchJanuary 25, 2022Alex J Whale, Michelle King, Ryan M Hull, Felix Krueger, Jonathan HouseleyIsothermal amplification buffer (NEB), 3 μl 100 mM MgSO4, 2 μl 10 mM dNTPs and 1 μl Bst 2 WarmStart DNA polymerase (NEB M0538S) was added and sample incubated 30 min at 65°C before precipitation with 12.5 μl 10 M NH4OAc, 1 μl GlycoBlue,

(14) A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosisNature CommunicationsDecember 1, 2021Guanhua Xun, Stephan Thomas Lane, Vassily Andrew Petrov, Brandon Elliott Pepa, Huimin Zhao(www.neb.com/protocols/2014/10/09/typical-rt-lamp-protocol) with following changes: WarmStart® Bst 2.0 (Cat# M0538L, NEB) with the final concentration of 320 units/mL, WarmStart® RTx (Cat# M0380L, NEB) with the final

(15) Single-copy detection of somatic variants from solid and liquid biopsyScientific ReportsDecember 1, 2021Ana-Luisa Silva, Paulina Klaudyna Powalowska, Magdalena Stolarek, Eleanor Ruth Gray, Rebecca Natalie Palmer, Bram Herman, Cameron Alexander Frayling, Barnaby William Balmforthwas prepared by mixing 3 µL Thermopol buffer (B9004, New England Biolabs), 0.4 µL BST 2.0 WarmStart (M0538, New England Biolabs), 1.2 µL dNTPs mix (10 mM stock, Promega), 1.13 µL Syto82 dye (S11363, Thermo Fisher Scientific

(16) Kouprey (Bos sauveli) genomes unveil polytomic origin of wild Asian BosiScienceNovember 19, 2021Mikkel-Holger S. Sinding, Marta M. Ciucani, Jazmín Ramos-Madrigal, Alberto Carmagnini, Jacob Agerbo Rasmussen, Shaohong Feng, Guangji Chen, Filipe G. Vieira, Valeria Mattiangeli, Rajinder K. Ganjoo, et al.T4 DNA Polymerase,New England Biolabs Inc.,Cat# M0203L "BSt 2,0 warmstart polymerase",New England Biolabs Inc.,Cat# M0538L ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(17) A mapping platform for mitotic crossover by single-cell multi-omicsMethods in EnzymologyNovember 15, 2021Peter Chovanec, Yi Yin(FisherScientific, cat. no. 07-678-003)Bst 2.0 WarmStart® DNA Polymerase (New England Biolabs, cat. no. M0538)HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, cat. no. E2050S)SUPERase•In™ RNase

(18) A chemical-enhanced system for CRISPR-Based nucleic acid detectionBiosensors and BioelectronicsNovember 15, 2021Zihan Li, Wenchang Zhao, Shixin Ma, Zexu Li, Yingjia Yao, Teng Feiwith 1 μL 10x isothermal amplification buffer, 1.4 mM of dNTPs, 6.5 mM MgSO4, 3.2 U Bst 2.0 (NEB, #M0538S), 3 U WarmStart RTx Reverse Transcriptase (NEB, #M0380S), 0.2 μM F3/B3 primers, 1.6 μM FIP/BIP primers,

(19) A Novel Miniature CRISPR-Cas13 System for SARS-CoV-2 DiagnosticsACS Synthetic BiologyOctober 15, 2021Ahmed Mahas, Qiaochu Wang, Tin Marsic, Magdy M. Mahfouz2 mM MgSO4, and 0.1% Tween 20) (B0537, NEB); 1.4 mM dNTPs; 8 U of Bst2.0 WarmStart DNA Polymerase (M0538, NEB); 7.5 U of WarmStart RTx Reverse Transcriptase (M0380, NEB); and 6 mM MgSO4 (B1003, NEB) in 25

(20) Intrinsic signal amplification by type III CRISPR-Cas systems provides a sequence-specific SARS-CoV-2 diagnosticCell Reports MedicineJune 15, 2021Andrew Santiago-Frangos, Laina N. Hall, Anna Nemudraia, Artem Nemudryi, Pushya Krishna, Tanner Wiegand, Royce A. Wilkinson, Deann T. Snyder, Jodi F. Hedges, Calvin Cicha, et al.RNase Inhibitor,NEB,Cat# M0314 Calcein,MP Biomedical,Cat# 02190167-CF WarmStart Bst 2.0,NEB,Cat# M0538L WarmStart RTx Reverse Transcriptase,NEB,Cat# M0380L Hi-T7 RNA Polymerase,NEB,Cat# M0658 TaqPath 1-Step RT-qPCR

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